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Addgene inc plasmid constructs
Plasmid Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mscv+ires+gfp/MSCV-beta-catenin-IRES-GFP+(Plasmid+%2314717)/pm41931626-315-9-16
Average 93 stars, based on 13 article reviews

plasmid constructs - by Bioz Stars, 2026-09

93/100 stars

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Plasmid Preparation:

Article Title: FOXP3 expression diversifies the metabolic capacity and enhances the efficacy of CD8 T cells in adoptive immunotherapy of melanoma
Article Snippet: pCL-Eco was a gift from Inder Verma1 (Addgene plasmid # 12371). .. MSCV-IRES-Gfp was a gift from Tannishtha Reya (Addgene plasmid # 20672). .. MSCV-IRES-Thy1.1 DEST2 was a gift from Anjana Rao (Addgene plasmid # 17442).

Article Title: Inhibition of heme biosynthesis triggers cuproptosis in acute myeloid leukaemia
Article Snippet: .. To generate MIG-IDH2 R140Q the human IDH2 R140Q was cloned into MSCV-IRES-GFP (Addgene plasmid #20672). ..

Article Title: Inhibition of heme biosynthesis triggers cuproptosis in acute myeloid leukemia.
Article Snippet: Article Inhibition of heme biosynthesis triggers cuproptosis in acute myeloid leukemia

Article Title: Lrp10 suppresses IL7R limiting CD8 T cell homeostatic expansion and anti-tumor immunity.
Article Snippet: .. MSCV-IRES-GFP was a gift from Tannishtha Reya, Addgene plasmid # 20672. .. Adoptive transfer experiments and in vivo CD8 T-cell functional assays For homeostatic proliferation experiments, recipient mice were sub-lethally irradiated with 6 Gy given as a single dose 24 h prior to cell transfer (X-RAD 320, Precision X-ray).

Clone Assay:

shRNA:

Article Title: Two independent translocation modes drive neural stem cell dissemination into the human fetal cortex
Article Snippet: .. The following plasmids were used in this study: MSCV-IRES-GFP (Tannishtha Reya, Addgene 20672); VSVG (a gift from P. Benaroch), Human EZR shRNA (TF308420, Origene), Human STK10 shRNA (TF320540, Origene), Human SLK shRNA (TG320620, Origene), Human DYNC1H1 shRNA (TL313335, Origene), Human RDX shRNA (TL309884, Origene), Human MSN shRNA (TL311375, Origene), Human ECT2 shRNA (TL304854, Origene), Human VIM shRNA (TL308419, Origene), Human PAFAH1B1 (LIS1) shRNA (TL310628, Origene), Dominant Negative KASH. .. Antibodies used in this study were mouse anti-SOX2 (Abcam Ab79351, 1/500), sheep anti-EOMES (R&D Sytems AF6166, 1/500), rabbit anti-NEUN (Abcam Ab177487, 1/500), chicken anti-GFP (Abcam Ab13970, 1/500), mouse anti-pVimentin (Abcam Ab22651, 1/1000), rabbit anti-NeuroD2 (Abcam, ab104430, 1/500), rabbit anti-HOPX (Proteintech, 11419-1-AP, 1/500), rabbit anti-NESPRIN2 (Abcam ab204308, 1/500), rabbit anti-PH3 (Abcam ab47297, 1/2000), mouse anti-gamma Tubulin (Sigma-Aldrich T5326, 1/1000), rabbit anti-pERM (Cell Signalling Technology, 3141S), mouse anti-LIFR (Abcam 89792, 1/500), rabbit anti-PTPRZ1 (Sigma HPA015103 Atlas antibodies, 1/500).

Dominant Negative Mutation:

Retroviral:

Article Title: RACK1 enhances STAT3 stability and promotes T follicular helper cell development and function during blood-stage Plasmodium infection in mice
Article Snippet: .. MSCV-IRES-GFP and pMKO.1GFP retroviral vectors were obtained from Addgene (plasmids #20672, #10676), the pCL-Eco packaging vector was provided by Dr. Lin Sun (Renji Hospital, Shanghai, China). .. For gene overexpression, total RNA of CD4 + T cells from day 5-infected Gnb2l1 fl/fl (WT) mice was isolated, reverse transcribed using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific), and PCR amplified using Q5 High-Fidelity DNA Polymerase (M0491, NEB).

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